Journal: Nature Communications
Article Title: Protection against overfeeding-induced weight gain is preserved in obesity but does not require FGF21 or MC4R
doi: 10.1038/s41467-024-45223-0
Figure Lengend Snippet: a – d Blood glucose ( a , n = 5 per group), plasma insulin ( b , n = 5 per group), plasma leptin ( c , n = 5 per group), and plasma ghrelin ( d , n = 4 Control d14, n = 6 Control d14 + 3, n = 2 ExpOF d14, n = 3 ExpOF d14 + 3) levels at different time points during the overfeeding intervention. e Fgf21 expression in eWAT, iWAT, iBAT, and liver ( n = 6). f Plasma FGF21 levels ( n = 6). g Schematic overview of the ExpOF setup in chow-fed FGF21 KO mice. Created with BioRender.com. h Body weight changes (percentage over baseline) in control ( n = 4) and ExpOF ( n = 5) FGF21 KO mice. Body weight is set at 100% at d0 (start of ExpOF). i Absolute body weight of same mice shown in h on day 0, 14, and 21. j Daily total (light pink for control mice, dark pink for ExpOF mice) and voluntary (purple for ExpOF mice) energy intake of same mice shown in h . k Heatmap showing average changes of top 20 regulated proteins (sorted by adjusted p value) between control and ExpOF mice at d14 and d14 + 3 ( n = 6). Heatmap shows fold-changes in normalized protein expression relative to control d14 mice. Red indicates high relative protein abundance and blue indicates low relative protein abundance. Regulated proteins (FDR < 0.1) are highlighted in bold. l – o Effects on food intake ( l , n ), and body weight ( m , o ) in C57BL/6 J DIO male mice injected once with 1 mg/kg of DLL1 ( l , m ) or LGMN ( n , o ) human recombinant proteins or vehicle ( n = 8 per group). p , q Open field test running traces ( p ) and total distance ( q ) of chow-fed lean C57BL6/J mice injected with LGMN (1 mg/kg) or vehicle ( n = 10 per group). Data shown as mean ± SEM with individual values plotted ( d – f , I , m , o , q ). P values were calculated using 2-way ANOVA using overfeeding and time as factors ( a – f , h – j , l , n ), or unpaired two-tailed Welch’s t test ( m , o , q ). */**/*** was used when p < 0.05, p < 0.01, p < 0.001, respectively, in Welch’s t test or post hoc comparison after ANOVA ( a – c , i , l – o ). */#/$; **/##/$$; ***/###/$$$ were used when p < 0.05; p < 0.01; p < 0.001, for overfeeding/time/interaction effects, respectively ( d – f ). ns: non-significant. n : number of mice (biological replicates). Source data are provided as a Source Data file.
Article Snippet: DLL1 and LGMN human recombinant protein were obtained from Sino Biological (#11635-H08H) and Cusabio (#EP012903HU), respectively.
Techniques: Expressing, Injection, Recombinant, Two Tailed Test, Comparison
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